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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
29/07/2022 |
Actualizado : |
02/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIANNITTI, F.; ARÁOZ, V.; SILVEIRA, C.S.; FRANCIA, M.E.; ROBELLO, C.; CABRERA, A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA E. FRANCIA, Laboratorio de Biología de Apicomplejos, Institut Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Unidad de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
Título : |
A Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157 |
DOI : |
10.3389/fvets.2022.889157 |
Idioma : |
Inglés |
Notas : |
Article history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. |
Contenido : |
Abstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. MenosAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a... Presentar Todo |
Palabras claves : |
ABORTION; DAIRY CATTLE; GENETIC DIVERSITY; Molecular epidemiology; Multilocus microsatellite typin; Neospora caninum; PATHOLOGY; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; REPRODUCTIVE DISEASES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16659/1/fvets-09-889157.pdf
https://www.frontiersin.org/articles/10.3389/fvets.2022.889157/full
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Marc : |
LEADER 03260naa a2200325 a 4500 001 1063470 005 2022-09-02 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fvets.2022.889157$2DOI 100 1 $aGIANNITTI, F. 245 $aA Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. 520 $aAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. 653 $aABORTION 653 $aDAIRY CATTLE 653 $aGENETIC DIVERSITY 653 $aMolecular epidemiology 653 $aMultilocus microsatellite typin 653 $aNeospora caninum 653 $aPATHOLOGY 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aREPRODUCTIVE DISEASES 700 1 $aARÁOZ, V. 700 1 $aSILVEIRA, C.S. 700 1 $aFRANCIA, M.E. 700 1 $aROBELLO, C. 700 1 $aCABRERA, A. 773 $tFrontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
Fecha actual : |
09/11/2015 |
Actualizado : |
08/03/2018 |
Tipo de producción científica : |
Actividades de Difusión |
Autor : |
JORNADA ANUAL SOJA, 1988, CIAAB-EEE, TREINTA Y TRES, UY.; CHEBATAROFF, N.; DEAMBROSI, E.; ZORRILLA DE SAN MARTÍN, G.; ACEVEDO, A.; PÉREZ, W.; ALTAMIRANO, A.; ÁLVAREZ, C.; CAYSSIALS, R.; DILANDRO, E. |
Afiliación : |
NICOLÁS CHEBATAROFF; ENRIQUE GERMAN DEAMBROSI CHURRUT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GONZALO ROBERTO ZORRILLA DE SAN MARTÍN PEREYRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANTONIO MARIA ACEVEDO VEGA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Soja: Resultados Experimentales 1987-88. |
Fecha de publicación : |
1988 |
Fuente / Imprenta : |
Treinta y Tres (Uruguay): CIAAB; EEE, 1988. |
Páginas : |
37 p. |
Serie : |
(CIAAB Resultados Experimentales; 20). |
Idioma : |
Español |
Notas : |
Centro de Investigaciones Agrícolas "Alberto Boerger" (CIAAB), Estación Experimental del Este (EEE). |
Contenido : |
Mejoramiento genético. Ensayos regionales de fertilización. Servicio de semillas. Investigación en inoculantes. Aptitud de suelos para el cultivo de soja: relevamiento realizado en la zona Este. |
Thesagro : |
SOJA. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5131/1/Soja-1987-1988.pdf
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Marc : |
LEADER 01045nam a2200265 a 4500 001 1058221 005 2018-03-08 008 1988 bl uuuu u00u1 u #d 100 1 $aJORNADA ANUAL SOJA, 1988, CIAAB-EEE, TREINTA Y TRES, UY. 245 $aSoja$bResultados Experimentales 1987-88.$h[electronic resource] 260 $aTreinta y Tres (Uruguay): CIAAB; EEE$c1988 300 $a37 p. 490 $a(CIAAB Resultados Experimentales; 20). 500 $aCentro de Investigaciones Agrícolas "Alberto Boerger" (CIAAB), Estación Experimental del Este (EEE). 520 $aMejoramiento genético. Ensayos regionales de fertilización. Servicio de semillas. Investigación en inoculantes. Aptitud de suelos para el cultivo de soja: relevamiento realizado en la zona Este. 650 $aSOJA 700 1 $aCHEBATAROFF, N. 700 1 $aDEAMBROSI, E. 700 1 $aZORRILLA DE SAN MARTÍN, G. 700 1 $aACEVEDO, A. 700 1 $aPÉREZ, W. 700 1 $aALTAMIRANO, A. 700 1 $aÁLVAREZ, C. 700 1 $aCAYSSIALS, R. 700 1 $aDILANDRO, E.
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